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Veterinary Focus

Issue number 26.1 Nutrition

Canine colostrum

Published 19/03/2021

Written by Sylvie Chastant-Maillard and Hanna Mila

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The neonatal period is a major risk period in the dog, since approximately 20% of live-born puppies die before they are 21 days old; 70% of deaths are in the first week post-partum.

Canine colostrum

Key points

Colostrum is crucial for puppy survival, providing both immunoglobulins and nutrition for the newborn.

The immunoglobulin concentration in colostrum in the first two days post-partum is five times greater than milk, but the levels drop very quickly with time.

Colostrum’s immunological quality varies from one dam to another, and also in the same dog between teats. The teats offering the highest-quality colostrum will also vary between bitches.

Monitoring growth over the first two days of life is a good indicator for predicting puppy survival in the neonatal period.

There is currently no complete (energy + immunity) substitute for canine colostrum.


The neonatal period is a major risk period in the dog, since approximately 20% of live-born puppies die before they are 21 days old; 70% of deaths are in the first week post-partum 12. Puppy survival within the early weeks is particularly dependent on colostrum, a specific secretion of the mammary gland produced during the first two days post-partum. Colostrum is both a source of nutrients and a source of immunoglobulins (Ig), as puppies are almost agammaglobulinemic at birth. The risk of neonatal mortality therefore depends on two factors: the quality of the transfer of passive immunity (evaluated by circulating IgG levels at 2 days of age) and the growth of the puppy between birth and 2 days old (at worst, weight loss should not be more than 4% of birth weight) 34. The immunity and energy supplied to the puppy by colostrum is therefore essential, but there is no guarantee that all puppies in a litter will receive sufficient colostrum; at two days of age, about 20% of puppies have a passive immunity deficit and 30% show insufficient early growth 34

Colostrum formation and composition

Colostrum is the first mammary secretion produced after delivery (and is occasionally present before parturition), with the transition to milk occurring between day two and three of lactation (Table 1). The actual quantity of colostrum produced by a lactating bitch is unknown.

Table 1. A comparison of colostrum and milk composition in the lactating bitch (5 and unpublished data).
Days of lactation
1 3 7 14 21
Nutrients Colostrum Milk Milk Milk Milk
Proteins (g/L) 143.0 102.3 81.7 66.8 68.4
Immunoglobulin G (g/L) 24.8 * 5.9 0.6 0.6
Lipids (g/L) 132.2 137.2 132.1 118.5 112.5
Lactose (g/L) 16.6 29.3 35.4 39.9 39.4
Calcium (mg/L) 1,363 1,366 1,773 1,950 1,929
Phosphorus (mg/L) 935 914 1,166 1,175 1,359
Energy (kcal/L) 1,831 1,761 1,657 1,493 1,444

* value unknown

During gestation, the mammary tissue develops under the influence of estrogens and progesterone, and secretion – induced by prolactin – is only possible when progesterone levels drop. Some colostrum compounds are synthesized by the epithelial mammary cells (proteins, lactose, lipids) while others, such as immunoglobulins (Ig), white blood cells, hormones and certain growth factors, are taken from the maternal bloodstream. Macroscopically, colostrum is yellowish and more viscous than milk. Qualitatively speaking, it is distinguished from milk essentially by its high protein concentration (twice that of milk secreted two weeks post-partum, being especially rich in immunoglobulins), a slightly higher lipid concentration (10% more) and a lower carbohydrate content (half that of milk) 56. For various reasons (a paucity of studies, considerable variability between dogs, and disparity in analytical methods), measurement of key components of colostrum differ between studies; protein levels are typically between 4-14%, lipid levels between 6-13%, and carbohydrate levels between 1.7-2.3% (578 and unpublished data).

In addition to casein (60% of total proteins), immunoglobulins represent between 20-37% of colostral proteins 67910. Three classes of immunoglobulin (IgG, IgM and IgA) are present in canine colostrum (with IgG, at 60-75% of the total, predominating) whilst IgE is undetectable. IgG in the colostrum is initially around 15-30 g/L but it falls very quickly, dropping to ~5 g/L on day 7 and less than 1 g/L on day 14 (unpublished data). The IgG concentration in milk is therefore 20 times less than that of colostrum. IgA represents 16-40% of colostral Ig but subsequently becomes the most common immunoglobulin in milk 710 (Figure 1). Most IgG comes from the mother’s serum, although a small fraction is produced locally in the mammary gland 11. The mammary gland is responsible for concentrating IgG, such that colostral levels are typically 3 times higher than in the maternal bloodstream, although there is no relation between the colostral concentration of IgG and the maternal serum concentration 1012. This selective concentration is under endocrine control, with the Ig stored in the mammary alveoli until its release after parturition 13. On the other hand, most IgA and IgM appear to be produced locally in the mammary gland by lymphocytes 13.

Immunoglobulin levels in the colostrum and milk

Figure 1. Immunoglobulin levels in the colostrum and milk; IgG, IgA and IgM were recorded from the mammary secretions of six Rottweiler dams 7.

Trypsin inhibitors are also found in the colostrum (but not in the milk), reducing degradation of colostral Ig and potentially increasing its absorption by the newborn 14. Colostrum also contains antimicrobial factors (such as lactoferrin and lysozyme), hormones (cortisol, thyroxine, insulin and growth hormone) and growth factors (e.g., insulin-like growth factors, epidermal growth factor and nerve growth factor 15. These are involved in the development and maturation of various organs such as the thyroid and intestines, as well as being vital for general puppy growth (see below).

Canine colostrum has high levels of two enzymes, gamma-glutamyl transferase and alkaline phosphatase, respectively 100 times and 10 times more concentrated than in maternal serum 16. These two chemicals are essentially absent from the circulating blood at birth, and so detection of these enzymes in a puppy’s serum will confirm ingestion of colostrum (although the enzyme levels do not correlate to the IgG concentration). 

Finally, canine colostrum also contains various cells including macrophages, neutrophils and lymphocytes. These cells are absorbed by the puppy before the intestinal barrier closes, and either enter the circulation, or play a role in cellular, humoral or local digestive immunity 17.

Roles of canine colostrum

Immunological protection 

The endotheliochorial placenta in dogs is almost completely impermeable to large-sized molecules such as immunoglobulins. This explains why puppies are born with low circulating levels (around 0.3 g/L) of IgG, as opposed to 8-25 g/L in adult dogs 31819. Colostral intake allows the acquisition of passive immunity, such that a neonate’s IgG serum concentration will be in the order of 6 g/L 48 hours after ingesting colostrum; 85-95% of a puppy’s circulating Ig is thus of colostral origin 20. The provision of Ig, which is potentiated by colostral anti-trypsins, is the most specific role for colostrum and is the determining factor for puppy survival (3), as most neonate mortality is due to infection 21. The colostral lactoferrin seems to play a marginal role in a puppy’s immunity 22 while the role of immune cells contained in colostrum is still not well defined. For passive immunity to be acquired, puppies must receive colostrum within the first eight hours of life (Figure 2). This time frame is critical for two reasons: 
  • Firstly, colostral IgG decreases rapidly in the first few hours post-partum.
  • Secondly, the rapid closure of the intestinal barrier; this is the point at which macromolecules (including IgG) can no longer cross the intestinal wall to enter the bloodstream, so that while a puppy absorbs ~ 40% of ingested colostral IgG at birth, only 20% is absorbed four hours after delivery and 9% twelve hours after delivery. 24 hours post-partum, absorption is nil 20.
It is important to encourage early colostral intake – within the first eight hours of birth – for optimal transfer of passive immunity

Figure 2. It is important to encourage early colostral intake – within the first eight hours of birth – for optimal transfer of passive immunity. © Chloé Robic, ENVT 


The immunological quality of the colostrum, in terms of IgG concentration, is quite variable, both between female dogs and between teat pairs of the same female (Figure 3). In one study looking at the colostrum of 44 female dogs from 13 different breeds in a single breeding kennel, the IgG levels varied between females by a factor of 5; neither the dam’s age or breed size, nor the litter size, appeared to influence the colostrum’s immunological quality 12. The IgG concentration in 180 samples from different teat pairs varied between 0.8 and 61 g/L, with a variation coefficient of 42% between teat pairs of the same bitch 12. However, the teat pair producing the highest-quality colostrum varies from one animal to another, so there is no value in advising puppies should suckle from one particular teat. Nevertheless, the marked variation in immunological quality between dams (and between the teats of the same female) may mean that certain litters have an increased risk of neonatal mortality. 

Immunological quality of colostrum according to number of teat pairs

Figure 3. Immunological quality of colostrum according to number of teat pairs 12.

Box plot of the IgG concentration of the colostrum in 44 female dogs. Each box represents 1st and 3rd quartiles (25th and 75th percentiles, or 50% of the population studied). The bar in each box represents the median, and the whiskers represent the 1st and 9th decile (10th and 9th percentile). M1 indicates the axillary teat pair and M5 the inguinal pair. The medians are not significantly different – indicating only one pair number does not systematically produce colostrum of higher immunological quality – but the whiskers are very long, reflecting the large variability between the same teat pairs in different dogs.

The colostrum supplies most of the IgG for systemic immunity, while IgA ensures local and digestive immunity, and in particular mucosal immunity. Colostral IgA is involved in local defense of the digestive tract and this role is continued with the ingestion of milk, which is rich in IgA. Other than the fraction absorbed into the bloodstream before closure of the intestinal barrier, IgA is distributed throughout both digestive and non-digestive mucosal sites 2023

Although transfer of maternal Ig helps reduce neonatal mortality, at the end of the pediatric period, when a puppy is 6-8 weeks of age, maternal immunity can interfere with puppy vaccination. The higher the IgG concentration acquired by two days of age, the higher it is during the pediatric period 24, and this increases the risk that a puppy may not be protected after vaccination. However, the interference is variable, being dependent on the individual animal, the immunogenicity of the vaccine, and the dose given.


Puppies have low reserves of adipose tissue at birth, and have limited glycogenolysis ability. The early energy supply from colostrum is therefore indispensable; growth is only possible if the energy supplied exceeds the puppy’s maintenance requirements (Figure 4).
The growth of a puppy over the first two days of life has a direct impact on its chances of survival
Figure 4. The growth of a puppy over the first two days of life has a direct impact on its chances of survival. Weight loss should not exceed 4% of birth weight. © Reproduction, ENVT 

The energy value of colostrum is at least 20% greater than milk, although the energy content can vary between dams (albeit within a fairly small range, by a factor of 1.6) and there can be slight differences between teat pairs of the same dog (a variation coefficient of around 8%, as opposed to 42% for the immunological value). Age, breed and litter size have not been shown to affect the energy value. 52% of the energy supplied by the colostrum is protein and 40% comes from lipids; variations in the energy value are principally explained by variations in the lipid levels 25.

Whilst the immunoglobulins and energy supplied from colostrum influence the risk of puppy mortality during the neonatal period 34 it is interesting to note that the immunological quality and energy value of colostrum are not correlated 28. In addition, the quantity of average colostrum that must be ingested for satisfactory immunity is 1.3 mL per 100 g of puppy body weight (assuming the puppy’s IgG serum levels reach 2.3 g/L, with a digestive absorption rate of 40%, a hematocrit of 35%, and IgG levels in the colostrum of 20 g/L). In contrast, the average quantity of ingested colostrum required to cover energy needs is much higher, at 12 mL per 100 g of puppy body weight (energy need of 212 kcal/kg per day if the colostrum supplies 1800 kcal/L).

Although it seems much more difficult to cover the energy need than the immunological need, a slightly higher proportion of puppies are deficient in energy (30%) than in Ig (20%) (Figure 5). Whilst the threshold levels of colostral IgG and energy required to control neonatal mortality have been determined in some species, they are currently unknown for the dog.

Growth and transfer of passive immunity are key indicators for puppy survival

Figure 5. Growth and transfer of passive immunity are key indicators for puppy survival.

The graph represents data from 149 puppies; 18 died before day 21 (red dots) and 131 were alive at day 21 (green triangles). The thresholds for survival during the two first days of life are weight loss of less than 4% of birthweight (approximated here to a nil growth) and a serum IgG level at the age of two days above 2.3 g/L. 

Graph adapted from 3 and 4

Organ development 

In addition to growth, colostrum is also involved in the development and maturation of certain organs, in particular the digestive tract. This is linked to colostral hormones and growth factors. One study reported that puppies fed colostrum had gastrointestinal tracts 60-95% better developed when compared to puppies of the same body weight given a synthetic milk formulation 26, although other studies do not consistently observe this 27

Induction of colostrum production and release

Sometimes a dam produces little or no colostrum at birth or immediately post-partum. This agalactia may be as a result of premature birth, cesarean section, endotoxemia or malnutrition, but it is most commonly due to post-partum stress, particularly in primiparous females. A quiet, calm area should always be provided at whelping, and in some cases medication may be recommended for anxious dams to encourage colostrum production (Table 2).


Table 2. Medical and other treatments indicated for agalactia in the bitch.
Medication Effect Dosage
Acepromazine Tranquilization; favors release of prolactin and increases secretion of colostrum 0.1-0.2 mg/kg SC
Metoclopramide Release of prolactin 0.1-0.2 mg/kg PO or SC q8h
Aglepristone Reduces progesterone levels, and hence encourages prolactin release 15 mg/kg SC 59-60 days post-ovulation. Administration is only recommended 20-24h before cesarean section
Ocytocin Local action stimulating the release of colostrum, but not its production 0.5-2 I.U. SC q2h
Fenugreek or fennel supplementation Stimulates milk secretion but mechanism unknown
Oral administration; optimum dose 


Alternatives to colostrum

When the dam is absent or does not produce enough colostrum, it is essential to source a substitute in order to limit neonatal mortality. At the very least an energy supply must be provided for the puppies, but a supply of immunoglobulins is also desirable. The ideal scenario is to have another bitch who has given birth less than 2-3 days previously, and either make her adopt the puppies or draw colostrum from her to administer to the puppies. If an adoptive dam whelped more than 2-3 days previously, her milk will ensure sufficient energy supply (since the energy value of milk is only 20% less than that of colostrum), but the supply of IgG will be insufficient: milk contains only 1-2 g/L of IgG compared to 20 g/L in colostrum, so a puppy will require 13-26 mL of milk to supply the same quantity of IgG obtained from 1 mL of colostrum. Data is not available for puppies, but kittens fed with milk from a foster cat had no significant transfer of IgG 28. Formula milks are void of canine immunoglobulins and have an energy concentration of about 1 kcal/mL (i.e., half that of colostrum) 29 and so again ensure a nutritional supply but no immunological supply. 

Conversely, serum drawn from an adult dog will contain immunoglobulins, but at a concentration around 3 times less than that of colostrum, and will have only a limited energy value. Trials administering canine serum orally at birth to puppies deprived of colostrum showed an increase in circulating IgG, but at a much lower level than that obtained from standard colostrum 1819.

However, one study 18 showed that oral administration of canine serum at birth to puppies deprived of colostrum did result in reasonable IgG levels. This suggests that, in some puppies at least, administering serum may ensure that the minimum protective IgG concentration (i.e., 2.3 g/L) is reached.

Currently bovine colostrum as a source of heterologous immunoglobulins is of interest, as it is easy to collect and readily available, but it has yet to be evaluated in puppies for its immunological or nutritional value. Another source of abundant Ig is IgY (from immunized hen eggs), with recent work showing that serum containing specific antibodies against canine pathogens (E.coli and CPV2) obtained from hyper-immunized eggs can be administered to puppies to provide immunity; the authors trialed administration of oral IgY to puppies before closure of the intestinal barrier and obtained promising results in terms of overall health, with improved growth over the first three weeks of life (unpublished data). 

In the absence of an ideal substitute, the only solution currently available is to establish a colostrum bank, as currently practised for cattle and horses. Breeders can draw milk from a bitch on the second day post-partum (which ensures her own puppies have acquired passive immunity). Generally, milking a lactating bitch is easy; after cleaning the skin with a chlorhexidine-based soap the colostrum can be collected in small-volume plastic tubes and frozen (Figure 6). Small quantities of colostrum can then be thawed (at 37°C (98.6°F); under no circumstances should a microwave oven be used) as necessary and administered by bottle or feeding tube at a dose of 1.5 mL per 100 g of puppy body weight per day. 

If a dam cannot supply colostrum to her litter, colostrum can be drawn from another bitch between 24 and 48 hours after she has whelped
Figure 6. If a dam cannot supply colostrum to her litter, colostrum can be drawn from another bitch between 24 and 48 hours after she has whelped, ensuring her own litter has received immunity but before the Ig levels have dropped. © Reproduction, ENVT


Canine colostrum is a secretion with a very particular composition designed to meet a puppy’s specific needs – namely, provision of passive immunity, energy and certain factors required for organ growth and differentiation. The quantity of colostrum received may be a limiting factor in the survival of certain puppies of a litter, whilst the impact of maternal nutrition on the quantity and quality of colostrum produced remains to be explored. From a practical point of view the development of a colostral substitute or supplement which can provide immunological support effective against canine pathogens as well as an energy supply would constitute a crucial advance in controlling neonatal mortality in puppies.


The authors would like to thank all the people who contributed to improving knowledge on canine colostrum, particularly Karine Reynaud, Elie Marcheteau, Marie-Blanche Bertieri, Jennifer Anne, Maelys Martin, Milène Gonnier, Lisa Rossig and Stéphanie Coinus.


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Sylvie Chastant-Maillard

Sylvie Chastant-Maillard

Dr. Chastant obtained her veterinary diploma in 1990 from the National Veterinary School of Alfort (France), and was awarded her doctorate for research into pre-implantation of mammalian embryos in 1995 Read more

Hanna Mila

Hanna Mila

Hanna Mila, Ecole Nationale Vétérinaire de Toulouse, France Read more

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